Cloning and expression analysis of PtFATB gene encoding the acyl-acyl carrier protein thioesterase in Populus tomentosa Carr.
Identifieur interne : 003C24 ( Main/Exploration ); précédent : 003C23; suivant : 003C25Cloning and expression analysis of PtFATB gene encoding the acyl-acyl carrier protein thioesterase in Populus tomentosa Carr.
Auteurs : Zhou Zhou [République populaire de Chine] ; Deqiang Zhang ; Mengzhu LuSource :
- Journal of genetics and genomics = Yi chuan xue bao [ 1673-8527 ] ; 2007.
Descripteurs français
- KwdFr :
- ADN complémentaire (analyse), ADN complémentaire (génétique), ADN des plantes (analyse), Alignement de séquences (MeSH), Amorces ADN (MeSH), Clonage moléculaire (MeSH), Données de séquences moléculaires (MeSH), Expression des gènes (MeSH), Génome végétal (MeSH), Populus (génétique), Populus (métabolisme), RT-PCR (MeSH), Séquence d'acides aminés (MeSH), Séquence nucléotidique (MeSH), Thiolester hydrolases (génétique), Thiolester hydrolases (métabolisme).
- MESH :
- analyse : ADN complémentaire, ADN des plantes.
- génétique : ADN complémentaire, Populus, Thiolester hydrolases.
- métabolisme : Populus, Thiolester hydrolases.
- Alignement de séquences, Amorces ADN, Clonage moléculaire, Données de séquences moléculaires, Expression des gènes, Génome végétal, RT-PCR, Séquence d'acides aminés, Séquence nucléotidique.
English descriptors
- KwdEn :
- Amino Acid Sequence (MeSH), Base Sequence (MeSH), Cloning, Molecular (MeSH), DNA Primers (MeSH), DNA, Complementary (analysis), DNA, Complementary (genetics), DNA, Plant (analysis), Gene Expression (MeSH), Genome, Plant (MeSH), Molecular Sequence Data (MeSH), Populus (genetics), Populus (metabolism), Reverse Transcriptase Polymerase Chain Reaction (MeSH), Sequence Alignment (MeSH), Thiolester Hydrolases (genetics), Thiolester Hydrolases (metabolism).
- MESH :
- chemical , analysis : DNA, Complementary, DNA, Plant.
- chemical , genetics : DNA, Complementary, Thiolester Hydrolases.
- chemical , metabolism : Thiolester Hydrolases.
- chemical : DNA Primers.
- genetics : Populus.
- metabolism : Populus.
- Amino Acid Sequence, Base Sequence, Cloning, Molecular, Gene Expression, Genome, Plant, Molecular Sequence Data, Reverse Transcriptase Polymerase Chain Reaction, Sequence Alignment.
Abstract
Acyl-ACP thioesterases (FATs) terminates the fatty acid synthesis and allow the transport of fatty acids out of the plastids, which are the important determinants of cellular metabolism. FATB is a member of FAT enzymes that has been described previously in most of the plants. In silico cloning is a new method that utilizes the bioinformatics on the complete genome and available EST database. In this study, a full-length cDNA clone of PtFATB gene was isolated from Populus tomentosa using this approach. It is 1,450 bp in length and the open reading frame encodes a peptide of 421 amino acids. The predicted amino acid sequence shows significant homology with those from other plant species, which contain typical domains owned by FATB proteins. The transcripts of PtFATB were abundant in leaves, and less in roots detected by using semiquantitative RT-PCR. When the shoots were subjected to the stress treatments (cold, dry, NaCl) and ABA (Abscisic acid), the expression of PtFATB was only slightly reduced under the treatment of low temperature. This suggests that the expression of PtFATB is in a constitutive fashion. This study provides the basis not only for the identification and characterization of this gene but also for the improvement of cold tolerance by controlling the expression of the PtFATB gene in trees in near future.
DOI: 10.1016/S1673-8527(07)60028-9
PubMed: 17498624
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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Zhang</name></author><author><name sortKey="Lu, Mengzhu" sort="Lu, Mengzhu" uniqKey="Lu M" first="Mengzhu" last="Lu">Mengzhu Lu</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2007">2007</date><idno type="RBID">pubmed:17498624</idno><idno type="pmid">17498624</idno><idno type="doi">10.1016/S1673-8527(07)60028-9</idno><idno type="wicri:Area/Main/Corpus">003B56</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">003B56</idno><idno type="wicri:Area/Main/Curation">003B56</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">003B56</idno><idno type="wicri:Area/Main/Exploration">003B56</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Cloning and expression analysis of PtFATB gene encoding the acyl-acyl carrier protein thioesterase in Populus tomentosa Carr.</title><author><name sortKey="Zhou, Zhou" sort="Zhou, Zhou" uniqKey="Zhou Z" first="Zhou" last="Zhou">Zhou Zhou</name><affiliation wicri:level="1"><nlm:affiliation>Key Laboratory of Forest Silviculture of the State Forestry Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Forest Silviculture of the State Forestry Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Zhang, Deqiang" sort="Zhang, Deqiang" uniqKey="Zhang D" first="Deqiang" last="Zhang">Deqiang Zhang</name></author><author><name sortKey="Lu, Mengzhu" sort="Lu, Mengzhu" uniqKey="Lu M" first="Mengzhu" last="Lu">Mengzhu Lu</name></author></analytic><series><title level="j">Journal of genetics and genomics = Yi chuan xue bao</title><idno type="ISSN">1673-8527</idno><imprint><date 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FATB is a member of FAT enzymes that has been described previously in most of the plants. In silico cloning is a new method that utilizes the bioinformatics on the complete genome and available EST database. In this study, a full-length cDNA clone of PtFATB gene was isolated from Populus tomentosa using this approach. It is 1,450 bp in length and the open reading frame encodes a peptide of 421 amino acids. The predicted amino acid sequence shows significant homology with those from other plant species, which contain typical domains owned by FATB proteins. The transcripts of PtFATB were abundant in leaves, and less in roots detected by using semiquantitative RT-PCR. When the shoots were subjected to the stress treatments (cold, dry, NaCl) and ABA (Abscisic acid), the expression of PtFATB was only slightly reduced under the treatment of low temperature. This suggests that the expression of PtFATB is in a constitutive fashion. This study provides the basis not only for the identification and characterization of this gene but also for the improvement of cold tolerance by controlling the expression of the PtFATB gene in trees in near future.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">17498624</PMID><DateCompleted><Year>2009</Year><Month>05</Month><Day>11</Day></DateCompleted><DateRevised><Year>2007</Year><Month>05</Month><Day>14</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">1673-8527</ISSN><JournalIssue CitedMedium="Print"><Volume>34</Volume><Issue>3</Issue><PubDate><Year>2007</Year><Month>Mar</Month></PubDate></JournalIssue><Title>Journal of genetics and genomics = Yi chuan xue bao</Title><ISOAbbreviation>J Genet Genomics</ISOAbbreviation></Journal><ArticleTitle>Cloning and expression analysis of PtFATB gene encoding the acyl-acyl carrier protein thioesterase in Populus tomentosa Carr.</ArticleTitle><Pagination><MedlinePgn>267-73</MedlinePgn></Pagination><Abstract><AbstractText>Acyl-ACP thioesterases (FATs) terminates the fatty acid synthesis and allow the transport of fatty acids out of the plastids, which are the important determinants of cellular metabolism. 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PubStatus="received"><Year>2006</Year><Month>01</Month><Day>19</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2006</Year><Month>04</Month><Day>18</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2007</Year><Month>5</Month><Day>15</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2009</Year><Month>5</Month><Day>12</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2007</Year><Month>5</Month><Day>15</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">17498624</ArticleId><ArticleId IdType="pii">S1673-8527(07)60028-9</ArticleId><ArticleId IdType="doi">10.1016/S1673-8527(07)60028-9</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>République populaire de Chine</li></country><settlement><li>Pékin</li></settlement></list><tree><noCountry><name sortKey="Lu, Mengzhu" sort="Lu, Mengzhu" uniqKey="Lu M" first="Mengzhu" last="Lu">Mengzhu Lu</name><name sortKey="Zhang, Deqiang" sort="Zhang, Deqiang" uniqKey="Zhang D" first="Deqiang" last="Zhang">Deqiang Zhang</name></noCountry><country name="République populaire de Chine"><noRegion><name sortKey="Zhou, Zhou" sort="Zhou, Zhou" uniqKey="Zhou Z" first="Zhou" last="Zhou">Zhou Zhou</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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